| Abstract: | Reversed-phase isolation and ion-exchange purification were combined in the automated solid-phase extraction of two polar s-triazine metabolites, 2-amino-4-chloro-6-(isopropylamino)-s-triazine (deethylatrazine) and 2-amino-4-chloro-6-(ethylamino)-s-triazine (deisopropylatrazine) from clay-loam and slit-loam soils and sandy aquifer sediments. First, methanol/ water (4/1, v/v) soil extracts were transferred to an automated workstation following evaporation of the methanol phase for the rapid reversed-phase isolation of the metabolites on an octadecylresin (C18). The retention of the triazine metabolites on C18 decreased substantially when trace methanol concentrations (1%) remained. Furthermore, the retention on C18 increased with decreasing aqueous solubility and increasing alkyl-chain length of the metabolites and parent herbicides, indicating a reversed-phase interaction. The analytes were eluted with ethyl acetate, which left much of the soil organic-matter impurities on the resin. Second, the small-volume organic eluate was purified on an anion-exchange resin (0.5 mL/min) to extract the remaining soil pigments that could foul the ion source of the GC/MS system. Recoveries of the analytes were 75%, using deuterated atrazine as a surrogate, and were comparable to recoveries by soxhlet extraction. The detection limit was 0.1 ??g/kg with a coefficient of variation of 15%. The ease and efficiency of this automated method makes it viable, practical technique for studying triazine metabolites in the environment. |
| Genre: | Article |
| ProdID: | 70016843 |
| Citation Author: | Mills, M. S.; Thurman, E. M. |
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| Citation End Page: | 1990 |
| Citation Issue: | 17 |
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| Citation Language: | English |
| Citation Larger Work Title: | Analytical Chemistry |
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| Citation Number Of Pages: | 6 |
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| Citation Search Results Text: | Mixed-mode isolation of triazine metabolites from soil and aquifer sediments using automated solid-phase extraction; 1992; Article; Journal; Analytical Chemistry; Mills, M. S.; Thurman, E. M. |
| Citation Start Page: | 1985 |
| Citation Volume: | 64 |
| Citation Year: | 1992 |
| Type: | citation/reference |
| Text: | Mixed-mode isolation of triazine metabolites from soil and aquifer sediments using automated solid-phase extraction; 1992; Article; Journal; Analytical Chemistry; Mills, M. S.; Thurman, E. M. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| Date Other: | Wed, 1 Jan 1992 00:00 -0600 |
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