| Abstract: | Laboratory bioassays were conducted to determine the utility of peroxidase (POD) activity as a biochemical indicator of contaminant exposure in the aquatic plant Hydrilla verticillata, Royle. The plants were exposed to anthracene, sulfometuron methyl (Oust??), Cd2+, Cr6+, Cu2+, Mn2+, and Se4+ in concentration factors of 10. POD was extracted and measured by spectrophotometric assay. There was a significant increase in POD activity after a 5-d exposure to each of the chemicals at 1 mg/L. The optimum pH for POD activity after exposure to the chemicals was 5.5 to 6.0. The increase in POD was found to be dose dependent for each of the chemicals. The lowest concentration of chemical to induce a significant POD increase was 0.01 mg/L for anthracene, Oust, Cd, Cr, and Cu; 0.1 mg/L for Se; and 1.0 mg/L for Mn.Laboratory bioassays were conducted to determine the utility of peroxidase (POD) activity as a biochemical indicator of contaminant exposure in the aquatic plant Hydrilla verticillata, Royle. The plants were exposed to anthracene, sulfometuron methyl (Oust), Cd2+, Cr6+, Cu2+, Mn2+, and Se4+ in concentration factors of 10. POD was extracted and measured by spectrophotometric assay. There was a significant increase in POD activity after a 5-d exposure to each of the chemicals at 1 mg/L. The optimum pH for POD activity after exposure to the chemicals was 5.5 to 6.0. The increase in POD was found to be dose dependent for each of the chemicals. The lowest concentration of chemical to induce a significant POD increase was 0.01 mg/L for anthracene, Oust, Cd, Cr, and Cu: 0.1 mg/L for Se; and 1.0 mg/L for Mn. |
| Genre: | Article |
| ProdID: | 70017569 |
| Citation Author: | Byl, T. D.; Sutton, H. D.; Klaine, S. J. |
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| Citation End Page: | 515 |
| Citation Issue: | 3 |
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| Citation Language: | English |
| Citation Larger Work Title: | Environmental Toxicology and Chemistry |
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| Citation Number Of Pages: | 7 |
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| Citation Search Results Text: | Evaluation of peroxidase as a biochemical indicator of toxic chemical exposure in the aquatic plant Hydrilla verticillata, Royle; 1994; Article; Journal; Environmental Toxicology and Chemistry; Byl, T. D.; Sutton, H. D.; Klaine, S. J. |
| Citation Start Page: | 509 |
| Citation Volume: | 13 |
| Citation Year: | 1994 |
| Type: | citation/reference |
| Text: | Evaluation of peroxidase as a biochemical indicator of toxic chemical exposure in the aquatic plant Hydrilla verticillata, Royle; 1994; Article; Journal; Environmental Toxicology and Chemistry; Byl, T. D.; Sutton, H. D.; Klaine, S. J. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| Date Other: | Sat, 1 Jan 1994 00:00 -0600 |
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