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Evaluation of Immunoassay for the Determination of Pesticides at a Large-Scale Groundwater Contamination Site

ACS Symposium Series

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Abstract

Pesticide concentrations in ground water at Rocky Mountain Arsenal (RMA) near Denver, Colorado, were determined using solid-phase extraction (SPE) gas chromatography/mass spectrometry (GC/MS) procedures and enzyme-linked immunosorbent assay (ELISA) for cyclodiene insecticides and triazine herbicides. Matrix interferences resulted in inconclusive results for some GC/MS analyses due to baseline disturbances and co-elution, but ELISA analyses consistently gave definitive results in a minimum amount of time. ELISA was used initially as a screening method, and pesticide concentrations and plume extents identified by ELISA were confirmed by SPE-GC/MS. A high degree of correlation was seen between results from GC/MS and ELISA methods for the triazine herbicides (correlation coefficient (R2) = 0.99). All areas with high pesticide concentrations were found to be within the boundaries of RMA.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Evaluation of Immunoassay for the Determination of Pesticides at a Large-Scale Groundwater Contamination Site
Series title:
ACS Symposium Series
Volume
657
Year Published:
1996
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
ACS Symposium Series
First page:
221
Last page:
233
Number of Pages:
13