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Preparation, cryopreservation, and growth of cells prepared from the green turtle (Chelonia mydas)

Methods in Cell Science

By:
, , , and
DOI: 10.1023/A:1009716712646

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Abstract

Techniques are described for preparing, preserving, and growing cell cultures from 30 to 40-day old green turtle embryos (2.0-3.0 cm length) including cells derived from skeletal muscle, liver, heart, kidney, eye, lung, and brain. Acceptable growth of all cells occurred in all standard cell culture media tested, with optimum growth temperature near 30??C. These cell cultures will be used in the study of sea turtle viral diseases including fibropapillomatosis, which is currently epidemic in some green turtle populations.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Preparation, cryopreservation, and growth of cells prepared from the green turtle (Chelonia mydas)
Series title:
Methods in Cell Science
DOI:
10.1023/A:1009716712646
Volume
19
Issue:
3
Year Published:
1997
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Methods in Cell Science
First page:
161
Last page:
168
Number of Pages:
8