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Inhibition of methane oxidation by Methylococcus capsulatus with hydrochlorofluorocarbons and fluorinated methanes

Applied and Environmental Microbiology

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Abstract

The inhibition of methane oxidation by cell suspensions of Methylococcus capsulatus (Bath) exposed to hydrochlorofluorocarbon 21 (HCFC-21; difluorochloromethane [CHF2Cl]), HCFC-22 (fluorodichloromethane [CHFCl2]), and various fluorinated methanes was investigated. HCFC-21 inhibited methane oxidation to a greater extent than HCFC-22, for both the particulate and soluble methane monooxygenases. Among the fluorinated methanes, both methyl fluoride (CH3F) and difluoromethane (CH2F2) were inhibitory while fluoroform (CHF3) and carbon tetrafluoride (CF4) were not. The inhibition of methane oxidation by HCFC-21 and HCFC-22 was irreversible, while that by methyl fluoride was reversible. The HCFCs also proved inhibitory to methanol dehydrogenase, which suggests that they disrupt other aspects of C1 catabolism in addition to methane monooxygenase activity.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Inhibition of methane oxidation by Methylococcus capsulatus with hydrochlorofluorocarbons and fluorinated methanes
Series title:
Applied and Environmental Microbiology
Volume
63
Issue:
7
Year Published:
1997
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Applied and Environmental Microbiology
First page:
2952
Last page:
2956