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Physiological levels of testosterone kill salmonid leukocytes in vitro

General and Comparative Endocrinology

By:
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DOI: 10.1006/gcen.1996.6858

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Abstract

Adult spring chinook salmon (Oncorhynchus tshawytscha) elaborate high plasma concentrations of testosterone during sexual maturation, and these levels of testosterone have been shown to reduce the salmonid immune response in vitro. Our search for the mechanism of testosterone's immunosuppressive action has led to the characterization of an androgen receptor in salmonid leukocytes. In the present study we examined the specific effects that testosterone had on salmonid leukocytes. Direct counts of viable leukocytes after incubation with and without physiological levels of testosterone demonstrate a significant loss of leukocytes in cultures exposed to testosterone. At least 5 days of contact with testosterone was required to produce significant immunosuppression and addition of a 'conditioned media' (supernatant from proliferating lymphocytes not exposed to testosterone) did not reverse the immunosuppressive effects of testosterone. These data lead us to conclude that testosterone may exert its immunosuppressive effects by direct action on salmonid leukocytes, through the androgen receptor described, and that this action leads to the death of a significant number of these leukocytes.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Physiological levels of testosterone kill salmonid leukocytes in vitro
Series title:
General and Comparative Endocrinology
DOI:
10.1006/gcen.1996.6858
Volume
106
Issue:
1
Year Published:
1997
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
First page:
113
Last page:
119
Number of Pages:
7