| Abstract: | N-sodium-N-chloro-??-toluenesulfonamide (chloramine-T) effectively controls bacterial gill disease (BGD) in cultured fishes. BGD, a common disease of hatchery-reared salmonids, causes more fish losses than any other disease among these species. This study describes a liquid chromatographic (LC) method that is capable of direct, simultaneous analysis of chloramine-T and its primary degradation product, ??-toluenesulfonamide (??-TSA), in water. The procedure involves reversed-phase (C18) LC analysis with ion suppression, using 0.01M phosphate buffer at pH 3. The mobile phase is phosphate buffer-acetonitrile (60 + 40) at 1 mL/min. Both chemicals can be detected with a UV spectrophotometer at 229 nm; the method is linear up to 40 mg chloramine-T or ??-TSA/L. Mean recoveries were 96.4 ?? 6.1% for water samples fortified with 0.03 mg chloramine-T/L and 95.3 ?? 4.6% for water samples fortified with 0.005 mg ??-TSA/L. Limits of detection without sample enrichment for chloramine-T and ??-TSA are 0.01 mg/L and 0.001 mg/L, respectively. |
| Genre: | Article |
| ProdID: | 70019974 |
| Citation Author: | Dawson, V. K.; Davis, R. A. |
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| Citation End Page: | 318 |
| Citation Issue: | 2 |
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| Citation Language: | English |
| Citation Larger Work Title: | Journal of AOAC International |
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| Citation Number Of Pages: | 3 |
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| Citation Search Results Text: | Liquid Chromatographic Determination of Chloramine-T and Its Primary Degradation Product, ??-Toluenesulfonamide, in Water; 1997; Article; Journal; Journal of AOAC International; Dawson, V. K.; Davis, R. A. |
| Citation Start Page: | 316 |
| Citation Volume: | 80 |
| Citation Year: | 1997 |
| Type: | citation/reference |
| Text: | Liquid Chromatographic Determination of Chloramine-T and Its Primary Degradation Product, ??-Toluenesulfonamide, in Water; 1997; Article; Journal; Journal of AOAC International; Dawson, V. K.; Davis, R. A. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| Date Other: | Wed, 1 Jan 1997 00:00 -0600 |
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