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Performance of serum-free broth media for growth of Renibacterium salmoninarum

Diseases of Aquatic Organisms

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Abstract

Growth of Renibacterium salmoninarum was compared in 14 different broth media; 13 serum-free, and 1 that contained newborn calf serum, KDM2+M. Supplementation with 1% v/v R. salmoninarum MCO4M metabolite was evaluated for 6 of the media that do not utilize it as part of their ingredients. Viable cells were enumerated on Days 10, 20, and 30 post inoculation to evaluate performance. The experiment was repeated 3 times using high, low, and medium (trials 1 to 3, respectively) cell concentrations as inoculum. In general there was no optimal medium and all performed well. The choice of which to employ depends on the ease of preparation and presence of certain ingredients that might affect subsequent assays. In trials 2 and 3, the pH was estimated using test papers at the same time as cells were counted. Maximum pH increase occurred with KDM2+M and those media containing charcoal. For most media, a simple pH determination could be used as a means to check that growth has occurred in a culture, particularly if charcoal was added directly to the media and a visual inspection could not be made to detect growth.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Performance of serum-free broth media for growth of Renibacterium salmoninarum
Series title:
Diseases of Aquatic Organisms
Volume
34
Issue:
1
Year Published:
1998
Language:
English
Publisher:
Inter-Research
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Diseases of Aquatic Organisms
First page:
21
Last page:
26