thumbnail

Liquid chromatographic determination of benzocaine and N-acetylbenzocaine in the edible fillet tissue from rainbow trout

Journal of Chromatography A

By:
, , , and
DOI: 10.1016/S0021-9673(99)00665-2

Links

Abstract

A method was developed for determining benzocaine and N-acetylbenzocaine concentrations in fillet tissue of rainbow trout. The method involves extracting the analytes with acetonitrile, removing lipids or hydrophobic compounds from the extract with hexane, and providing additional clean-up with solid-phase extraction techniques. Analyte concentrations are determined using reversed-phase high-performance liquid chromatographic techniques with an isocratic mobile phase and UV detection. The accuracy (range, 92 to 121%), precision (R.S.D., <14%), and sensitivity (method quantitation limit, <24 ng/g) for each analyte indicate the usefulness of this method for studies characterizing the depletion of benzocaine residues from fish exposed to benzocaine. Copyright (C) 1999.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Liquid chromatographic determination of benzocaine and N-acetylbenzocaine in the edible fillet tissue from rainbow trout
Series title:
Journal of Chromatography A
DOI:
10.1016/S0021-9673(99)00665-2
Volume
855
Issue:
1
Year Published:
1999
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Journal of Chromatography A
First page:
255
Last page:
260
Number of Pages:
6