| Abstract: | Freshwater-adapted killifish (Fundulus heteroclitus) were transferred directly from soft fresh water to full-strength sea water for periods of 1h, 3h, 8h and 1, 2, 7, 14 and 30 days. Controls were transferred to fresh water for 24 h. Measured variables included: blood [Na+], osmolality, glucose and cortisol levels, basal and stimulated rates of ion transport and permeability of in vitro opercular epithelium, gill Na+/K+-ATPase and citrate synthase activity and chloride cell ultrastructure. These data were compared with previously published killifish cystic fibrosis transmembrane conductance regulator (kfCFTR) expression in the gills measured over a similar time course. Plasma cortisol levels peaked at 1 h, coincident with a rise in plasma [Na+]. At 8 h after transfer to sea water, a time at which previous work has shown kfCFTR expression to be elevated, blood osmolality and [Na+] were high, and cortisol levels and opercular membrane short-circuit current (I(SC); a measure of Cl- secretion rate) were low. The 24h group, which showed the highest level of kfCFTR expression, had the highest plasma [Na+] and osmolality, elevated plasma cortisol levels, significantly lower opercular membrane resistance, an increased opercular membrane ion secretion rate and collapsed tubule inclusions in mitochondria-rich cells, but no change in gill Na+/K+-ATPase and citrate synthase activity or plasma glucose levels. Apparently, killifish have a rapid (<1h) cortisol response to salinity coupled to subsequent (8-48 h) expression of kfCFTR anion channel proteins in existing mitochondria-rich cells that convert transport from ion uptake to ion secretion. |
| Genre: | Article |
| ProdID: | 70021444 |
| Citation Author: | Marshall, W. S.; Emberley, T. R.; Singer, T. D.; Bryson, S. E.; McCormick, S. D. |
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| Citation End Page: | 1544 |
| Citation Issue: | 11 |
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| Citation Language: | English |
| Citation Larger Work Title: | Journal of Experimental Biology |
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| Citation Number Of Pages: | 10 |
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| Citation Search Results Text: | Time course of salinity adaptation in a strongly euryhaline estuarine teleost, fundulus heteroclitus: A multivariable approach; 1999; Article; Journal; Journal of Experimental Biology; Marshall, W. S.; Emberley, T. R.; Singer, T. D.; Bryson, S. E.; McCormick, S. D. |
| Citation Start Page: | 1535 |
| Citation Volume: | 202 |
| Citation Year: | 1999 |
| Type: | citation/reference |
| Text: | Time course of salinity adaptation in a strongly euryhaline estuarine teleost, fundulus heteroclitus: A multivariable approach; 1999; Article; Journal; Journal of Experimental Biology; Marshall, W. S.; Emberley, T. R.; Singer, T. D.; Bryson, S. E.; McCormick, S. D. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| Date Other: | Fri, 1 Jan 1999 00:00 -0600 |
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