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In situ detection of the Clostridium botulinum type C1 toxin gene in wetland sediments with a nested PCR assay

Applied and Environmental Microbiology

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, , and

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Abstract

A nested PCR was developed for detection of the Clostridium botulinum type C1 toxin gene in sediments collected from wetlands where avian botulism outbreaks had or had not occurred. The C1 toxin gene was detected in 16 of 18 sites, demonstrating both the ubiquitous distribution of C. botulinum type C in wetland sediments and the sensitivity of the detection assay.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
In situ detection of the Clostridium botulinum type C1 toxin gene in wetland sediments with a nested PCR assay
Series title:
Applied and Environmental Microbiology
Volume
65
Issue:
7
Year Published:
1999
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Applied and Environmental Microbiology
First page:
3240
Last page:
3243
Number of Pages:
4