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Sequence features and phylogenetic analysis of the stress protein Hsp90?? in chinook salmon (Oncorhynchus tshawytscha), a poikilothermic vertebrate

Biochemical and Biophysical Research Communications

By:
, , and
DOI: 10.1006/bbrc.1999.0707

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Abstract

We cloned and sequenced a chinook salmon Hsp90 cDNA; sequence analysis shows it to be Hsp90??. Phylogenetic analysis supports the hypothesis that ?? and ?? paralogs of Hsp90 arose as a result of a gene duplication event and that they diverged early in the evolution of vertebrates, before tetrapods separated from the teleost lineage. Among several differences distinguishing poikilothermic Hsp90?? sequences from their bird and mammal orthologs, the teleost versions specifically lack a characteristic QTQDQP phosphorylation site near the N-terminus. We used the cDNA to develop an RNA (Northern) blot to quantify cellular Hsp90 mRNA levels. Chinook salmon embryonic (CHSE-214) cells responded to heat shock with a rapid rise in Hsp90 mRNA through 4 h, followed by a gradual decline over the next 20 h. Hsp90 mRNA level may be useful as a stress indicator, especially in a laboratory setting or in response to acute heat stress.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Sequence features and phylogenetic analysis of the stress protein Hsp90?? in chinook salmon (Oncorhynchus tshawytscha), a poikilothermic vertebrate
Series title:
Biochemical and Biophysical Research Communications
DOI:
10.1006/bbrc.1999.0707
Volume
258
Issue:
3
Year Published:
1999
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Biochemical and Biophysical Research Communications
First page:
784
Last page:
791
Number of Pages:
8