| Abstract: | Induction of triploidy and tetraploidy in Nile tilapia, Oreochromis niloticus, was investigated by heat shock, cold shock, hydrostatic pressure, and/ or chemicals (cytochalasin A, B, and D). Additionally, efficacy of combined protocols was determined. Heat shock 10 min after fertilization induced triploidy when incubation temperature was 24 C but not when incubation temperature was 31 C. Heat shock of 40-41 C at 4-6 min after fertilization was effective in inducing up to 100% triploidy with hatchability similar to controls. Cold shock at 13 C for 45 min five min after fertilization induced 85-100% triploids. Heat shock and multiple heat shocking were the most effective treatments for the induction of tetraploidy. Two heat treatments of 41 C applied at 65 and 80 min after fertilization for 5 min each produced approximately 80% tetraploidy in hatched fry. Immersion of fertilized eggs in cytochalasin A, B, or D at concentrations up to 10 ??g/L applied at various times and durations was ineffective in inducing triploidy or tetraploidy. |
| Genre: | Article |
| ProdID: | 70021681 |
| Citation Author: | El, Gamal, A. -R. A.; Davis, K. B.; Jenkins, J. A.; Les, Torrans, E. |
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| Citation End Page: | 275 |
| Citation Issue: | 2 |
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| Citation Language: | English |
| Citation Larger Work Title: | Journal of the World Aquaculture Society |
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| Citation Number Of Pages: | 7 |
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| Citation Search Results Text: | Induction of triploidy and tetraploidy in Nile tilapia, Oreochromis niloticus (L.); 1999; Article; Journal; Journal of the World Aquaculture Society; El, Gamal, A. -R. A.; Davis, K. B.; Jenkins, J. A.; Les, Torrans, E. |
| Citation Start Page: | 269 |
| Citation Volume: | 30 |
| Citation Year: | 1999 |
| Type: | citation/reference |
| Text: | Induction of triploidy and tetraploidy in Nile tilapia, Oreochromis niloticus (L.); 1999; Article; Journal; Journal of the World Aquaculture Society; El, Gamal, A. -R. A.; Davis, K. B.; Jenkins, J. A.; Les, Torrans, E. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| Date Other: | Fri, 1 Jan 1999 00:00 -0600 |
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