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Induction of triploidy and tetraploidy in Nile tilapia, Oreochromis niloticus (L.)

Journal of the World Aquaculture Society

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Abstract

Induction of triploidy and tetraploidy in Nile tilapia, Oreochromis niloticus, was investigated by heat shock, cold shock, hydrostatic pressure, and/ or chemicals (cytochalasin A, B, and D). Additionally, efficacy of combined protocols was determined. Heat shock 10 min after fertilization induced triploidy when incubation temperature was 24 C but not when incubation temperature was 31 C. Heat shock of 40-41 C at 4-6 min after fertilization was effective in inducing up to 100% triploidy with hatchability similar to controls. Cold shock at 13 C for 45 min five min after fertilization induced 85-100% triploids. Heat shock and multiple heat shocking were the most effective treatments for the induction of tetraploidy. Two heat treatments of 41 C applied at 65 and 80 min after fertilization for 5 min each produced approximately 80% tetraploidy in hatched fry. Immersion of fertilized eggs in cytochalasin A, B, or D at concentrations up to 10 ??g/L applied at various times and durations was ineffective in inducing triploidy or tetraploidy.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Induction of triploidy and tetraploidy in Nile tilapia, Oreochromis niloticus (L.)
Series title:
Journal of the World Aquaculture Society
Volume
30
Issue:
2
Year Published:
1999
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
First page:
269
Last page:
275
Number of Pages:
7