Induction of triploidy and tetraploidy in Nile tilapia, Oreochromis niloticus (L.)
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Abstract
Induction of triploidy and tetraploidy in Nile tilapia, Oreochromis niloticus, was investigated by heat shock, cold shock, hydrostatic pressure, and/or chemicals (cytochalasin A, B, and D). Additionally, efficacy of combined protocols was determined. Heat shock 10 min after fertilization induced triploidy when incubation temperature was 24 C but not when incubation temperature was 31 C. Heat shock of 40–41 C at 4–6 min after fertilization was effective in inducing up to 100% triploidy with hatchability similar to controls. Cold shock at 13 C for 45 min five min after fertilization induced 85–100% triploids. Heat shock and multiple heat shocking were the most effective treatments for the induction of tetraploidy. Two heat treatments of 41 C applied at 65 and 80 min after fertilization for 5 min each produced approximately 80% tetraploidy in hatched fry. Immersion of fertilized eggs in cytochalasin A, B, or D at concentrations up to 10 μg/L applied at various times and durations was ineffective in inducing triploidy or tetraploidy.
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Induction of triploidy and tetraploidy in Nile tilapia, Oreochromis niloticus (L.) |
| Series title | Journal of the World Aquaculture Society |
| DOI | 10.1111/j.1749-7345.1999.tb00875.x |
| Volume | 30 |
| Issue | 2 |
| Year Published | 1999 |
| Language | English |
| Publisher | World Aquaculture Society |
| Contributing office(s) | National Wetlands Research Center |
| Description | 7 p. |
| First page | 269 |
| Last page | 275 |
| Google Analytic Metrics | Metrics page |