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Preliminary study of gill NA+,K+-ATPase activity in juvenile spring chinook salmon following electroshock or handling stress

North American Journal of Fisheries Management

By:
, ,
DOI: 10.1577/1548-8675(2000)020<0528:PSOGNK>2.3.CO;2

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Abstract

We compared gill Na+,K+-ATPase in subyearling and yearling spring chinook salmon Oncorhynchus tshawytscha 3 h, 24 h, and 7 d after exposure to either a short pulsed DC electroshock (300 V, 50 Hz, 8-ms pulse duration) or an acute handling stress. Mean gill Na+,K+-ATPase values ranged from 7.5 to 11.8 ??mol inorganic phosphate (Pi) ?? (mg protein)-1 ?? h-1. No significant differences were detected, with the exception of electroshocked subyearlings 7 d after treatment. Increased activity was attributed to the presence of two influential values. No significant differences were detected after removal of these observations, so the increase was not considered biologically significant. Inclusion of the outliers did not alter our interpretation of the results given that the observed increase was slight compared with the magnitude of changes reported under experimental conditions and in migrating juvenile salmonids. The treatment groups underwent a typical stress response and had significantly elevated cortisol and glucose levels 3 h after treatment. Recovery to control levels occurred within 24 h for cortisol and from 24 h to 7 d for glucose. Our results lead to the conclusion that neither acute electroshock nor acute handling stress alters Na+,K+-ATPase activity in juvenile spring chinook salmon.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Preliminary study of gill NA+,K+-ATPase activity in juvenile spring chinook salmon following electroshock or handling stress
Series title:
North American Journal of Fisheries Management
DOI:
10.1577/1548-8675(2000)020<0528:PSOGNK>2.3.CO;2
Volume
20
Issue:
2
Year Published:
2000
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
First page:
528
Last page:
533
Number of Pages:
6