Integration of lithologic logs, geophysical logs, and hydraulic tests is critical in characterizing heterogeneous aquifers. Typically only a limited number of aquifer tests can be performed, and these need to be designed to provide hydraulic properties for the principle aquifers in the system. This study describes the integration of logs and aquifer tests in the development of a hydrostratigraphic model for the surficial aquifer system in and around Big Cypress National Preserve in eastern Collier County, Florida. Borehole flowmeter tests provide qualitative permeability profiles in most of 26 boreholes drilled in the Study area. Flow logs indicate the depth of transmissive units, which are correlated across the study area. Comparison to published studies in adjacent areas indicates that the main limestone aquifer of the 000000Tamiami Formation in the study area corresponds with the gray limestone aquifer in western Dade County and the water table and lower Tamiami Aquifer in western Collier County. Four strategically located, multiwell aquifer tests are used to quantify the qualitative permeability profiles provided by the flowmeter log analysis. The hydrostratigraphic model based on these results defines the main aquifer in the central part of the study area as unconfined to semiconfined with a transmissivity as high as 30,000 m2/day. The aquifer decreases in transmissivity to less than 10,000 m2/day in some parts of western Collier County, and becomes confined to the east and northeast of the study area, where transmissivity decreases to below 5000 m2/day.Integration of lithologic logs, geophysical logs, and hydraulic tests is critical in characterizing heterogeneous aquifers. Typically only a limited number of aquifer tests can be performed, and these need to be designed to provide hydraulic properties for the principle aquifers in the system. This study describes the integration of logs and aquifer tests in the development of a hydrostratigraphic model for the surficial aquifer system in and around Big Cypress National Preserve in eastern Collier County, Florida. Borehole flowmeter tests provide qualitative permeability profiles in most of 26 boreholes drilled in the study area. Flow logs indicate the depth of transmissive units, which are correlated across the study area. Comparison to published studies in adjacent areas indicates that the main limestone aquifer of the Tamiami Formation in the study area corresponds with the gray limestone aquifer in western Dade County and the water table and lower Tamiami Aquifer in western Collier County. Four strategically located, multiwell aquifer tests are used to quantify the qualitative permeability profiles provided by the flowmeter log analysis. The hydrostratigraphic model based on these results defines the main aquifer in the central part of the study area as unconfined to semiconfined with a transmissivity as high as 30,000 m2/day. The aquifer decreases in transmissivity to less than 10,000 m2/day in some parts of western Collier County, and becomes confined to the east and northeast of the study area, where transmissivity decreases to below 5000 m2/day.