| Abstract: | Under methanogenic conditions, microbial degradation of [1,2-14C]vinyl chloride (VC) resulted in significant (14 ?? 3% maximum recovery) but transient recovery of radioactivity as 14C-acetate. Subsequently, 14C- acetate was degraded to 14CH4 and 14CO2 (18 ?? 2% and 54 ?? 3% final recoveries, respectively). In contrast, under 2-bromoethanesulfonic acid (BES) amended conditions, 14C-acetate recovery remained high (27 ?? 1% maximum recovery) throughout the study, no 14CH4 was produced, and the final recovery of 14CO2 was only 35 ?? 4%. These results demonstrate that oxidative acetogenesis may be an important mechanism for anaerobic VC biodegradation. Moreover, these results (1) demonstrate that microbial degradation of VC to CH4 and CO2 may involve oxidative acetogenesis followed by acetotrophic methanogenesis and (2) suggest that oxidative acetogenesis may be the initial step in the net oxidation of VC to CO2 reported previously under Fe(III)-reducing, SO4-reducing, and humic acids- reducing conditions.Under methanogenic conditions, microbial degradation of [1,2-14C]vinyl chloride (VC) resulted in significant (14 ?? 3% maximum recovery) but transient recovery of radioactivity as 14C-acetate. Subsequently, 14C-acetate was degraded to 14CH4 and 14CO2 (18 ?? 2% and 54 ?? 3% final recoveries respectively). In contrast, under 2-bromoethanesulfonic acid (BES) amended conditions, 14C-acetate recovery remained high (27 ?? 1% maximum recovery) throughout the study, no 14CH4 was produced, and the final recovery of 14CO2 was only 35 ?? 4%. These results demonstrate that oxidative acetogenesis may be an important mechanism for anaerobic VC biodegradation. Moreover, these results (1) demonstrate that microbial degradation of VC to CH4 and CO2 may involve oxidative acetogenesis followed by acetotrophic methanogenesis and (2) suggest that oxidative acetogenesis may be the initial step in the net oxidation of VC to CO2 reported previously under Fe(III)-reducing, SO4-reducing, and humic acids-reducing conditions. |
| Genre: | Article |
| ProdID: | 70022544 |
| Citation Author: | Bradley, P. M.; Chapelle, F. H. |
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| Citation End Page: | 2763 |
| Citation Issue: | 13 |
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| Citation Language: | English |
| Citation Larger Work Title: | Environmental Science and Technology |
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| Citation Number Of Pages: | 3 |
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| Citation Publisher: | ACS |
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| Citation Search Results Text: | Acetogenic microbial degradation of vinyl chloride; 2000; Article; Journal; Environmental Science and Technology; Bradley, P. M.; Chapelle, F. H. |
| Citation Start Page: | 2761 |
| Citation Volume: | 34 |
| Citation Year: | 2000 |
| Type: | citation/reference |
| Text: | Acetogenic microbial degradation of vinyl chloride; 2000; Article; Journal; Environmental Science and Technology; Bradley, P. M.; Chapelle, F. H. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| URL (DIGITAL OBJECT IDENTIFIER): | http://dx.doi.org/10.1021/es991371m |
| Date Other: | Sat, 1 Jan 2000 00:00 -0600 |
| Publisher: | ACS |
