| Abstract: | The rapid activation of gill Na+,K+-ATPase was analyzed in the mummichog (Fundulus heteroclitus) and Atlantic salmon (Salmo salar) transferred from low salinity (0.1 ppt) to high salinity (25-35 ppt). In parr and presmolt, Salmo salar gill Na+,K+-ATPase activity started to increase 3 days after transfer. Exposure of Fundulus heteroclitus to 35 ppt seawater (SW) induced a rise in gill Na+,K+-ATPase activity 3 hr after transfer. After 12 hr, the values dropped to initial levels but showed a second significant increase 3 days after transfer. The absence of detergent in the enzyme assay resulted in lower values of gill Na+,K+-ATPase, and the rapid increase after transfer to SW was not observed. Na+,K+-ATPase activity of gill filaments in vitro for 3 hr increased proportionally to the osmolality of the culture medium (600 mosm/kg > 500 mosm/kg > 300 mosm/kg). Osmolality of 800 mosm/kg resulted in lower gill Na+,K+-ATPase activity relative to 600 mosm/kg. Increasing medium osmolality to 600 mosm/kg with mannitol also increased gill Na+,K+-ATPase. Cycloheximide inhibited the increase in gill Na+,K+-ATPase activity observed in hyperosmotic medium in a dose-dependent manner (10-4 M > 10-5 M > 10-6 M). Actinomycin D or bumetanide in the culture (doses of 10-4 M, 10-5 M, and 10-6 M) did not affect gill Na+,K+-ATPase. Injection of fish with actinomycin D prior to gill organ culture, however, prevented the increase in gill Na+,K+-ATPase activity in hyperosmotic media. The results show a very rapid and transitory increase in gill Na+,K+-ATPase activity in the first hours after the transfer of Fundulus heteroclitus to SW that is dependent on translational and transcriptional processes. (C) 2000 Wiley-Liss, Inc. |
| Genre: | Article |
| ProdID: | 70022574 |
| Citation Author: | Mancera, J. M.; McCormick, S. D. |
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| Citation End Page: | 274 |
| Citation Issue: | 4 |
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| Citation Language: | English |
| Citation Larger Work Title: | Journal of Experimental Zoology |
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| Citation Number Of Pages: | 12 |
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| Citation Search Results Text: | Rapid activation of gill Na+,K+-ATPase in the euryhaline teleost Fundulus heteroclitus; 2000; Article; Journal; Journal of Experimental Zoology; Mancera, J. M.; McCormick, S. D. |
| Citation Start Page: | 263 |
| Citation Volume: | 287 |
| Citation Year: | 2000 |
| Type: | citation/reference |
| Text: | Rapid activation of gill Na+,K+-ATPase in the euryhaline teleost Fundulus heteroclitus; 2000; Article; Journal; Journal of Experimental Zoology; Mancera, J. M.; McCormick, S. D. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| URL (DIGITAL OBJECT IDENTIFIER): | http://dx.doi.org/10.1002/1097-010X(20000901)287:4<263::AID-JEZ1>3.0.CO;2-I |
| Date Other: | Sat, 1 Jan 2000 00:00 -0600 |
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