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Detection of Pesticides and Pesticide Metabolites Using the Cross Reactivity of Enzyme Immunoassays

Journal of AOAC International

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Abstract

Enzyme immunoassay is an important environmental analysis method that may be used to identify many pesticide analytes in water samples. Because of similarities in chemical structure between various members of a pesticide class, there often may be an unwanted response that is characterized by a percentage of cross reactivity. Also, there may be cross reactivity caused by degradation products of the target analyte that may be present in the sample. In this paper, the concept of cross reactivity caused by degradation products or by nontarget analytes is explored as a tool for identification of metabolites or structurally similar compounds not previously known to be present in water samples. Two examples are examined in this paper from various water quality studies. They are alachlor and its metabolite, alachlor ethane sulfonic acid, and atrazine and its class members, prometryn and propazine. A method for using cross reactivity for the detection of these compounds is explained in this paper.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Detection of Pesticides and Pesticide Metabolites Using the Cross Reactivity of Enzyme Immunoassays
Series title:
Journal of AOAC International
Volume
84
Issue:
1
Year Published:
2001
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Journal of AOAC International
First page:
162
Last page:
167
Number of Pages:
6