Identification of Methyl Halide-Utilizing Genes in the Methyl Bromide-Utilizing Bacterial Strain IMB-1 Suggests a High Degree of Conservation of Methyl Halide-Specific Genes in Gram-Negative Bacteria

Woodall, C. A.; Warner, K. L.; Oremland, R. S.; Murrell, J. C.; Mcdonald, I. R.

Abstract:

Strain IMB-1, an aerobic methylotrophic member of the alpha subgroup of the Proteobacteria, can grow with methyl bromide as a sole carbon and energy source. A single cmu gene cluster was identified in IMB-1 that contained six open reading frames: cmuC, cmuA, orf146, paaE, hutI, and partial metF. CmuA from IMB-1 has high sequence homology to the methyltransferase CmuA from Methylobacterium chloromethanicum and Hyphomicrobium chloromethanicum and contains a C-terminal corrinoid-binding motif and an N-terminal methyl-transferase motif. However, cmuB, identified in M. chloromethanicum and H. chloromethanicum, was not detected in IMB-1.

Genre:

Article

ProdID:

70023619

Citation Author:

Woodall, C. A.; Warner, K. L.; Oremland, R. S.; Murrell, J. C.; Mcdonald, I. R.

Citation Contributing Office:

Citation Datum:

Citation Day:

Citation Edition:

Citation Editor:

Citation End Page:

1963

Citation Issue:

Citation Keywords:

Citation Language:

English

Citation Larger Work Title:

Applied and Environmental Microbiology

Citation LatN:

Citation LatS:

Citation LonE:

Citation LonW:

Citation Month:

Citation No Pagination:

Citation Number Of Pages:

Citation Online Only Flag:

Citation Phsyical Description:

Citation Projection:

Citation Public Comments:

Citation Publisher:

Citation Series:

Citation Series Code:

Citation Series Number:

Citation Search Results Text:

Identification of Methyl Halide-Utilizing Genes in the Methyl Bromide-Utilizing Bacterial Strain IMB-1 Suggests a High Degree of Conservation of Methyl Halide-Specific Genes in Gram-Negative Bacteria; 2001; Article; Journal; Applied and Environmental Microbiology; Woodall, C. A.; Warner, K. L.; Oremland, R. S.; Murrell, J. C.; Mcdonald, I. R.