thumbnail

Cloning, sequencing, and expression of interferon-?? from elk in North America

Journal of Wildlife Diseases

By:
, , and

Links

  • The Publications Warehouse does not have links to digital versions of this publication at this time
  • Download citation as: RIS

Abstract

Eradication of Mycobacterium bovis relies on accurate detection of infected animals, including potential domestic and wildlife reservoirs. Available diagnostic tests lack the sensitivity and specificity necessary for accurate detection, particularly in infected wildlife populations. Recently, an in vitro diagnostic test for cattle which measures plasma interferon-gamma (IFN-??) levels in blood following in vitro incubation with M. bovis purified protein derivative has been enveloped. This test appears to have increased sensitivity over traditional testing. Unfortunately, it does not detect IFN-?? from Cervidae. To begin to address this problem, the IFN-?? gene from elk (Cervus elaphus) was cloned, sequenced, expressed, and characterized. cDNA was cloned from mitogen stimulated peripheral blood mononuclear cells. The predicted amino acid (aa) sequence was compared to known sequences from cattle, sheep, goats, red deer (Cervus elaphus), humans, and mice. Biological activity of the recombinant elk IFN-?? (rElkIFN-??) was confirmed in a vesicular stomatitis virus cytopathic effect reduction assay. Production of monoclonal antibodies to IFN-?? epitopes conserved between ruminant species could provide an important tool for the development of reliable, practical diagnostic assays for detection of a delayed type hypersensitivity response to a variety of persistent infectious agents in ruminants, including M. bovis and Brucella abortus. Moreover, development of these reagents will aid investigators in studies to explore immunological responses of elk that are associated with resistance to infectious diseases.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Cloning, sequencing, and expression of interferon-?? from elk in North America
Series title:
Journal of Wildlife Diseases
Volume
37
Issue:
1
Year Published:
2001
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Journal of Wildlife Diseases
First page:
164
Last page:
171
Number of Pages:
8