Soluble scute proteins of healthy and ill desert tortoises (Gopherus agassizii)

Homer, B. L.; Li, C.; Berry, K. H.; Denslow, N. D.; Jacobson, E. R.; Sawyer, R. H.; Williams, J. E.

Abstract:

Objectives - To characterize protein composition of shell scute of desert tortoises and to determine whether detectable differences could be used to identify healthy tortoises from tortoises with certain illnesses. Animals - 20 desert tortoises. Procedures - Complete postmortem examinations were performed on all tortoises. Plastron scute proteins were solubilized, scute proteins were separated by use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and proteins were analyzed, using densitometry. Two-dimensional immobilized pH gradient-PAGE (2D IPG-PAGE) and immunoblot analysis, using polyclonal antisera to chicken-feather ?? keratin and to alligator-scale ?? keratin, were conducted on representative samples. The 14-kd proteins were analyzed for amino acid composition. Results - The SDS-PAGE and densitometry revealed 7 distinct bands, each with a mean relative protein concentration of > 1 %, ranging from 8 to 47 kd, and a major protein component of approximately 14 kd that constituted up to 75% of the scute protein. The 2D IPG-PAGE revealed additional distinct 62-and 68-kd protein bands. On immunoblot analysis, the 14-, 32-, and 45-kd proteins reacted with both antisera. The 14-kd proteins had an amino acid composition similar to that of chicken ?? keratins. There was a substantial difference in the percentage of the major 14-kd proteins from scute of ill tortoises with normal appearing shells, compared with 14-kd proteins of healthy tortoises. Conclusions and Clinical Relevance - The major protein components of shell scute of desert tortoises have amino acid composition and antigenic features of ?? keratins. Scute protein composition may be altered in tortoises with certain systemic illnesses.

Genre:

Article

ProdID:

70023683

Citation Author:

Homer, B. L.; Li, C.; Berry, K. H.; Denslow, N. D.; Jacobson, E. R.; Sawyer, R. H.; Williams, J. E.

Citation Contributing Office:

Citation Datum:

Citation Day:

Citation Edition:

Citation Editor:

Citation End Page:

110

Citation Issue:

Citation Keywords:

Citation Language:

English

Citation Larger Work Title:

American Journal of Veterinary Research

Citation LatN:

Citation LatS:

Citation LonE:

Citation LonW:

Citation Month:

Citation No Pagination:

Citation Number Of Pages:

Citation Online Only Flag:

Citation Phsyical Description:

Citation Projection:

Citation Public Comments:

Citation Publisher:

Citation Series:

Citation Series Code:

Citation Series Number:

Citation Search Results Text:

Soluble scute proteins of healthy and ill desert tortoises (Gopherus agassizii); 2001; Article; Journal; American Journal of Veterinary Research; Homer, B. L.; Li, C.; Berry, K. H.; Denslow, N. D.; Jacobson, E. R.; Sawyer, R. H.; Williams, J. E.