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Evaluation of USEPA method 1622 for detection of Cryptosporidium oocysts in stream waters

Journal - American Water Works Association

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Abstract

To improve surveillance for Cryptosporidium oocysts in water, the US Environmental Protection Agency developed method 1622, which consists of filtration, concentration, immunomagnetic separation, fluorescent antibody and 4, 6-diamidino-2-phenylindole (DAPI) counter-staining, and microscopic evaluation. Two filters were compared for analysis of 11 stream water samples collected throughout the United States. Replicate 10-L stream water samples (unspiked and spiked with 100-250 oocysts) were tested to evaluate matrix effects. Oocyst recoveries from the stream water samples averaged 22% (standard deviation [SD] = ??17%) with a membrane disk and 12% (SD = ??6%) with a capsule filter. Oocyst recoveries from reagent water precision and recovery samples averaged 39% (SD = ??13%) with a membrane disk and 47% (SD = ??19%) with a capsule filter. These results demonstrate that Cryptosporidium oocysts can be recovered from stream waters using method 1622, but recoveries are lower than those from reagent-grade water. This research also evaluated concentrations of indicator bacteria in the stream water samples. Because few samples were oocyst-positive, relationships between detections of oocysts and concentrations of indicator organisms could not be determined.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Evaluation of USEPA method 1622 for detection of Cryptosporidium oocysts in stream waters
Series title:
Journal - American Water Works Association
Volume
93
Issue:
1
Year Published:
2001
Language:
English
Publisher:
American Water Works Assoc
Publisher location:
Denver, CO, United States
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
First page:
78
Last page:
87
Number of Pages:
10