| Abstract: | To improve surveillance for Cryptosporidium oocysts in water, the US Environmental Protection Agency developed method 1622, which consists of filtration, concentration, immunomagnetic separation, fluorescent antibody and 4, 6-diamidino-2-phenylindole (DAPI) counter-staining, and microscopic evaluation. Two filters were compared for analysis of 11 stream water samples collected throughout the United States. Replicate 10-L stream water samples (unspiked and spiked with 100-250 oocysts) were tested to evaluate matrix effects. Oocyst recoveries from the stream water samples averaged 22% (standard deviation [SD] = ??17%) with a membrane disk and 12% (SD = ??6%) with a capsule filter. Oocyst recoveries from reagent water precision and recovery samples averaged 39% (SD = ??13%) with a membrane disk and 47% (SD = ??19%) with a capsule filter. These results demonstrate that Cryptosporidium oocysts can be recovered from stream waters using method 1622, but recoveries are lower than those from reagent-grade water. This research also evaluated concentrations of indicator bacteria in the stream water samples. Because few samples were oocyst-positive, relationships between detections of oocysts and concentrations of indicator organisms could not be determined. |
| Genre: | Article |
| ProdID: | 70023874 |
| Citation Author: | Simmons, III, O. D.; Sobsey, M. D.; Schaefer, III, F. W.; Francy, D. S.; Nally, R. A.; Heaney, C. D. |
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| Citation End Page: | 87 |
| Citation Issue: | 1 |
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| Citation Language: | English |
| Citation Larger Work Title: | Journal / American Water Works Association |
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| Citation Number Of Pages: | 10 |
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| Citation Publisher: | American Water Works Assoc |
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| Citation Search Results Text: | Evaluation of USEPA method 1622 for detection of Cryptosporidium oocysts in stream waters; 2001; Article; Journal; Journal / American Water Works Association; Simmons, III, O. D.; Sobsey, M. D.; Schaefer, III, F. W.; Francy, D. S.; Nally, R. A.; Heaney, C. D. |
| Citation Start Page: | 78 |
| Citation Volume: | 93 |
| Citation Year: | 2001 |
| Type: | citation/reference |
| Text: | Evaluation of USEPA method 1622 for detection of Cryptosporidium oocysts in stream waters; 2001; Article; Journal; Journal / American Water Works Association; Simmons, III, O. D.; Sobsey, M. D.; Schaefer, III, F. W.; Francy, D. S.; Nally, R. A.; Heaney, C. D. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| Date Other: | Mon, 1 Jan 2001 00:00 -0600 |
| Publisher: | American Water Works Assoc |
