| Abstract: | Polyhalogenated hydrocarbons have been implicated in the anomalous sexual differentiation of mammals and reptiles. Here, a temperature-sensitive turtle sex determination assay using the red-eared slider (Trachemys scripta elegans) was used to determine the estrogenic or antiestrogenic activity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and 3,3???,4,4???,5-pentachlorobiphenyl (PCB-126). Neither TCDD nor PCB-126 showed a statistically significant difference in the resulting sex ratios (Fisher‘s exact test, p < 0.45). As a consequence of the dosing technique (eggshell spotting), the shell retained 90 and 96% of the dose for PCB-126 and TCDD, respectively, similar to retention of estradiol-17??. However, the dosing allowed transfer of sufficient chemical to achieve tissue concentrations that were greater than most concentrations reported for environmentally incurred residues. Similar relative mass distributions of PCB-126 and TCDD were observed in albumin (14-20%), yolk (55-70%), and embryo (16-25%). Relative concentration distributions in the embryo approached those in the yolk, 37 to 40% and 40 to 52%, respectively, while relative concentrations in the albumin remained at 11 to 20%. Lipid-normalized TCDD and PCB-126 concentrations were 30- to 40-fold greater in the embryo than in the yolk. It is hypothesized that nonpassive partitioning processes may have occurred in the embryo. |
| Genre: | Article |
| ProdID: | 70024157 |
| Citation Author: | Gale, R. W.; Bergeron, J. M.; Willingham, E. J.; Crews, D. |
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| Citation End Page: | 2482 |
| Citation Issue: | 11 |
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| Citation Language: | English |
| Citation Larger Work Title: | Environmental Toxicology and Chemistry |
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| Citation Number Of Pages: | 6 |
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| Citation Search Results Text: | Turtle sex determination assay: Mass balance and responses to 2,3,7,8-tetrachlorodibenzo-p-dioxin and 3,3???,4,4???,5-pentachlorobiphenyl; 2002; Article; Journal; Environmental Toxicology and Chemistry; Gale, R. W.; Bergeron, J. M.; Willingham, E. J.; Crews, D. |
| Citation Start Page: | 2477 |
| Citation Volume: | 21 |
| Citation Year: | 2002 |
| Type: | citation/reference |
| Text: | Turtle sex determination assay: Mass balance and responses to 2,3,7,8-tetrachlorodibenzo-p-dioxin and 3,3???,4,4???,5-pentachlorobiphenyl; 2002; Article; Journal; Environmental Toxicology and Chemistry; Gale, R. W.; Bergeron, J. M.; Willingham, E. J.; Crews, D. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| Date Other: | Tue, 1 Jan 2002 00:00 -0600 |
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