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Isolation and characterization of microsatellite loci in the intertidal sponge Halichondria panicea

Molecular Ecology Notes

By:
, , , and
DOI: 10.1046/j.1471-8286.2003.00511.x

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Abstract

GA- and CA-enriched genomic libraries were constructed for the intertidal sponge Halichondria panicea. Unique repeat motifs identified varied from the expected simple dinucleotide repeats to more complex repeat units. All sequences tended to be highly repetitive but did not necessarily contain the targeted motifs. Seven microsatellite loci were evaluated on sponges from the clone source population. All seven were polymorphic with 5.43??0.92 mean number of alleles. Six of the seven loci that could be resolved had mean heterozygosities of 0.14-0.68. The loci identified here will be useful for population studies.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Isolation and characterization of microsatellite loci in the intertidal sponge Halichondria panicea
Series title:
Molecular Ecology Notes
DOI:
10.1046/j.1471-8286.2003.00511.x
Volume
3
Issue:
4
Year Published:
2003
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Molecular Ecology Notes
First page:
560
Last page:
562