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A rapid and efficient assay for extracting DNA from fungi

Letters in Applied Microbiology

By:
, , , and
DOI: 10.1046/j.1472-765x.2002.01071.x

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Abstract

Aims: A method for the rapid extraction of fungal DNA from small quantities of tissue in a batch-processing format was investigated. Methods and Results: Tissue (< 3.0 mg) was scraped from freshly-grown fungal isolates. The tissue was suspended in buffer AP1 and subjected to seven rounds of freeze/thaw using a crushed dry ice/ethanol bath and a boiling water bath. After a 30 min boiling step, the tissue was quickly ground against the wall of the microfuge tube using a sterile pipette tip. The Qiagen DNeasy Plant Tissue Kit protocol was then used to purify the DNA for PCR/ sequencing applications. Conclusions: The method allowed batch DNA extraction from multiple fungal isolates using a simple yet rapid and reliable assay. Significance and Impact of the Study: Use of this assay will allow researchers to obtain DNA from fungi quickly for use in molecular assays that previously required specialized instrumentation, was time-consuming or was not conducive to batch processing.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
A rapid and efficient assay for extracting DNA from fungi
Series title:
Letters in Applied Microbiology
DOI:
10.1046/j.1472-765x.2002.01071.x
Volume
34
Issue:
3
Year Published:
2002
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Letters in Applied Microbiology
First page:
210
Last page:
214
Number of Pages:
5