| Abstract: | The respiratory arsenate reductase from the Gram-positive, haloalkaliphile, Bacillus selenitireducens strain MLS10 was purified and characterized. It is a membrane bound heterodimer (150 kDa) composed of two subunits ArrA (110 kDa) and ArrB (34 kDa), with an apparent Km for arsenate of 34 ??M and Vmax of 2.5 ??mol min-1 mg-1. Optimal activity occurred at pH 9.5 and 150 g l-1 of NaCl. Metal analysis (inductively coupled plasma mass spectrometry) of the holoenzyme and sequence analysis of the catalytic subunit (ArrA; the gene for which was cloned and sequenced) indicate it is a member of the DMSO reductase family of molybdoproteins. ?? 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. |
| Genre: | Article |
| ProdID: | 70025235 |
| Citation Author: | Afkar, E.; Lisak, J.; Saltikov, C.; Basu, P.; Oremland, R. S.; Stolz, J. F. |
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| Citation End Page: | 112 |
| Citation Issue: | 1 |
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| Citation Language: | English |
| Citation Larger Work Title: | FEMS Microbiology Letters |
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| Citation Number Of Pages: | 6 |
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| Citation Search Results Text: | The respiratory arsenate reductase from Bacillus selenitireducens strain MLS10; 2003; Article; Journal; FEMS Microbiology Letters; Afkar, E.; Lisak, J.; Saltikov, C.; Basu, P.; Oremland, R. S.; Stolz, J. F. |
| Citation Start Page: | 107 |
| Citation Volume: | 226 |
| Citation Year: | 2003 |
| Type: | citation/reference |
| Text: | The respiratory arsenate reductase from Bacillus selenitireducens strain MLS10; 2003; Article; Journal; FEMS Microbiology Letters; Afkar, E.; Lisak, J.; Saltikov, C.; Basu, P.; Oremland, R. S.; Stolz, J. F. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| URL (DIGITAL OBJECT IDENTIFIER): | http://dx.doi.org/10.1016/S0378-1097(03)00609-8 |
| Date Other: | Wed, 1 Jan 2003 00:00 -0600 |
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