Although the fragmentation of the natural landscape of coastal southern California, USA, is accelerating, large-scale assessments of regional connectivity are lacking. Because of their large area requirements and long dispersal movements, mammalian carnivores can be effective focal species to use when evaluating landscape-level connectivity. Our goal was to make an initial assessment of the extent of landscape-level connectivity in coastal southern California using mountain lions (Felis concolor [Linnaeus]) and bobcats (Felis rufus [Shreber]) as focal species. We first characterized habitat preferences for mountain lions and bobcats from previously derived habitat relationship models for these species; the resulting maps provided a coarse view of habitat preferences for use at regional scales. We then constructed GIS models to evaluate the disturbance impact of roadways and development, major determinants of carnivore distribution and abundance in the south coast region. Finally, we combined the habitat relationship models with the disturbance impact models to characterize habitat connectivity for mountain lions and bobcats in the ecoregion. Habitat connectivity in the ecoregion appeared higher for bobcats than for mountain lions due in part to higher habitat suitability for bobcats in coastal lowland areas. Our models suggest that much of the key carnivore habitat in the coastal southern California is at risk; over 80% of high suitability habitat and over 90% of medium suitability habitat for carnivores is found in the least protected land management classes. Overall, these models allow for (1) identification of core habitat blocks for carnivores and key landscape connections between core areas, (2) evaluation of the level of protection of these areas, and (3) a regional framework within which to develop and coordinate local management and conservation plans.