thumbnail

A quantitative evaluation of two methods for preserving hair samples

Molecular Ecology Notes

By:
, ,
DOI: 10.1046/j.1471-8286.2003.00358.x

Links

Abstract

Hair samples are an increasingly important DNA source for wildlife studies, yet optimal storage methods and DNA degradation rates have not been rigorously evaluated. We tested amplification success rates over a one-year storage period for DNA extracted from brown bear (Ursus arctos) hair samples preserved using silica desiccation and -20 ??C freezing. For three nuclear DNA microsatellites, success rates decreased significantly after a six-month time point, regardless of storage method. For a 1000 bp mitochondrial fragment, a similar decrease occurred after a two-week time point. Minimizing delays between collection and DNA extraction will maximize success rates for hair-based noninvasive genetic sampling projects.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
A quantitative evaluation of two methods for preserving hair samples
Series title:
Molecular Ecology Notes
DOI:
10.1046/j.1471-8286.2003.00358.x
Volume
3
Issue:
1
Year Published:
2003
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
First page:
163
Last page:
166
Number of Pages:
4