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Identification of a genetic marker that discriminates ocean-type and stream-type chinook salmon in the Columbia River basin

Transactions of the American Fisheries Society

By:
, , , , and
DOI: 10.1577/1548-8659(2003)132<0131:IOAGMT>2.0.CO;2

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Abstract

A marker based on randomly amplified polymorphic DNA (RAPD), OT-38, was discovered that nonlethally discriminates between stream-type and ocean-type populations of chinook salmon Oncorhynchus tshawytscha in the Columbia River basin, including the threatened fall-run (ocean-type) and spring-run (stream-type) Snake River populations. This marker was developed by amplifying chinook salmon genomic DNA with a single RAPD primer, sequencing the termini of the polymorphic products, and designing primer pairs for allele-specific amplification. It was used to assay 18-80 individuals from several wild and hatchery populations differing in year-class, freshwater life history, and location along the Columbia River OT-38 unambiguously distinguished ocean-type from stream-type populations in 93.1% of the chinook salmon sampled.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Identification of a genetic marker that discriminates ocean-type and stream-type chinook salmon in the Columbia River basin
Series title:
Transactions of the American Fisheries Society
DOI:
10.1577/1548-8659(2003)132<0131:IOAGMT>2.0.CO;2
Volume
132
Issue:
1
Year Published:
2003
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Transactions of the American Fisheries Society
First page:
131
Last page:
142
Number of Pages:
12