Identification of a genetic marker that discriminates ocean-type and stream-type chinook salmon in the Columbia River basin
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Abstract
A marker based on randomly amplified polymorphic DNA (RAPD), OT-38, was discovered that nonlethally discriminates between stream-type and ocean-type populations of chinook salmon Oncorhynchus tshawytscha in the Columbia River basin, including the threatened fall-run (ocean-type) and spring-run (stream-type) Snake River populations. This marker was developed by amplifying chinook salmon genomic DNA with a single RAPD primer, sequencing the termini of the polymorphic products, and designing primer pairs for allele-specific amplification. It was used to assay 18-80 individuals from several wild and hatchery populations differing in year-class, freshwater life history, and location along the Columbia River OT-38 unambiguously distinguished ocean-type from stream-type populations in 93.1% of the chinook salmon sampled.
Study Area
| Publication type | Article |
|---|---|
| Publication Subtype | Journal Article |
| Title | Identification of a genetic marker that discriminates ocean-type and stream-type chinook salmon in the Columbia River basin |
| Series title | Transactions of the American Fisheries Society |
| DOI | 10.1577/1548-8659(2003)132<0131:IOAGMT>2.0.CO;2 |
| Volume | 132 |
| Issue | 1 |
| Year Published | 2003 |
| Language | English |
| Publisher | Taylor & Francis |
| Contributing office(s) | Western Fisheries Research Center |
| Description | 12 p. |
| First page | 131 |
| Last page | 142 |
| Country | United States |
| State | California, Oregon, Washginton |
| Other Geospatial | Columbia River Basin, Quinault River, Sacramento River |
| Online Only (Y/N) | N |
| Additional Online Files (Y/N) | N |
| Google Analytic Metrics | Metrics page |