| Abstract: | We established a method of directly detecting Clostridium botulinum type C cells, while minimizing spore detection, in the intestinal contents of Mozambique tilapia (Oreochromis mossambicus). This technique involved extraction of predominantly cellular DNA from tilapia intestinal tracts and used a polymerase chain reaction assay to detect presence of type C1 toxin gene. We consistently detected C. botulinum type C cells in tilapia gastrointestinal contents at a level of 7.5 ?? 10 4 cells per 0.25 g material or 1.9 ?? 10 3 cells. This technique is useful for determining prevalence of the potentially active organisms within a given population of fish and may be adapted to other types of C. botulinum and vertebrate populations as well. ?? Wildlife Disease Association 2004. |
| Genre: | Article |
| ProdID: | 70026502 |
| Citation Author: | Nol, P.; Williamson, J. L.; Rocke, T. E.; Yuill, T. M. |
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| Citation End Page: | 753 |
| Citation Issue: | 4 |
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| Citation Language: | English |
| Citation Larger Work Title: | Journal of Wildlife Diseases |
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| Citation Number Of Pages: | 5 |
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| Citation Search Results Text: | Detection of Clostridium botulinum type C cells in the gastrointestinal tracts of Mozambique tilapia (Oreochromis mossambicus) by polymerase chain reaction; 2004; Article; Journal; Journal of Wildlife Diseases; Nol, P.; Williamson, J. L.; Rocke, T. E.; Yuill, T. M. |
| Citation Start Page: | 749 |
| Citation Volume: | 40 |
| Citation Year: | 2004 |
| Type: | citation/reference |
| Text: | Detection of Clostridium botulinum type C cells in the gastrointestinal tracts of Mozambique tilapia (Oreochromis mossambicus) by polymerase chain reaction; 2004; Article; Journal; Journal of Wildlife Diseases; Nol, P.; Williamson, J. L.; Rocke, T. E.; Yuill, T. M. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| Date Other: | Thu, 1 Jan 2004 00:00 -0600 |
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