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Molecular characterization of birnaviruses isolated from wild marine fishes at the Flemish Cap (Newfoundland)

Diseases of Aquatic Organisms

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Abstract

Several isolates of aquatic birnaviruses were recovered from different species of wild fish caught in the Flemish Cap, a Newfoundland fishery close to the Atlantic coast of Canada. The nucleotide sequence of a region of the NS gene was identical among the isolates and was most similar to the Dry Mills and West Buxton reference strains of infectious pancreatic necrosis virus (IPNV). Phylogenetic analysis of the sequence of a region of the VP2 gene demonstrated that the isolates were most closely aligned with the American strains of IPNV serotype Al. Electron microscopy of virus structures clarified and concentrated from cultures of infected chinook salmon embryo (CHSE-214) cells revealed a majority of typical IPNV-like icosahedral particles, as well as a low proportion of type I tubules having a diameter of approximately 55 nm and a variable length of up to 2 ??m. The tubules could be propagated in cell cultures, but always in the presence of low proportions of icosahedral particles. Cloning of selected isolates by serial dilution yielded preparations with a high proportion of the tubular structures with a density in CsCl gradients of approximately 1.30 g cm-3. Polyacrylamide gel electrophoresis revealed the material in the band was composed of the IPNV pVP2 and VP2 proteins.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Molecular characterization of birnaviruses isolated from wild marine fishes at the Flemish Cap (Newfoundland)
Series title:
Diseases of Aquatic Organisms
Volume
61
Issue:
1-2
Year Published:
2004
Language:
English
Publisher:
Inter-Research
Description:
p.1-10
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Diseases of Aquatic Organisms
First page:
1
Last page:
10
Number of Pages:
10