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Development and composition of a mixed culture for bioremediation of chlorinated ethenes and ethanes

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Abstract

Microbial organisms capable of dechlorinating 1,1,2,2 tetrachloroethane (TeCA) and its chlorinated ethane and ethylene daughter products were enriched in surface sediments collected from the West Branch Canal Creek wetland area, leading to the formation of two mixed cultures using slightly different enrichment methods. Both WBC-1 and WBC-2 were capable of rapid and complete reductive dechlorination of TeCA and its daughter products (1,1,2-trichloroethane, 1,2-dichloroethane, trichloroethylene, 1,2-dichloroethylene, and vinyl chloride) to ethylene, and addition of either culture to wetland sediment and to engineered peat/compost mixtures resulted in significant enhancement of dechlorination. However, the WBC-2 culture supported better sustained activity and was more readily scaled up for application in bioaugmentation treatments, whereas dechlorination activity was gradually lost in WBC-1. The microbial composition of WBC-1 and WBC-2 were determined by cloning and sequencing 500 base pairs of the 16S rDNA gene and the methyl co-reductase. Methanogens identified in the consortia were members of the Order Methanomicrobiales, which includes acetoclastic methanogens. This is an abstract of a paper presented at the Proceedings of the 8th International In Situ and On-Site Bioremediation Symposium (Baltimore, MD 6/6-9/2005).

Additional Publication Details

Publication type:
Conference Paper
Publication Subtype:
Conference Paper
Title:
Development and composition of a mixed culture for bioremediation of chlorinated ethenes and ethanes
ISBN:
9781574771527
Volume
3
Year Published:
2005
Language:
English
First page:
1486
Last page:
1487
Number of Pages:
2
Conference Title:
8th International In Situ and On-Site Bioremediation Symposium
Conference Location:
Baltimore, MD
Conference Date:
6 June 2005 through 9 June 2005