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Detection of Helicobacter pylori and fecal indicator bacteria in five North American rivers.

Journal of Water and Health

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Abstract

This study examines the use of fecal indicator bacteria (FIB) as a predictor of the presence of Helicobacter spp. A combination of standard culture and molecular techniques were used to detect and quantify FIB, Helicobacter spp. and H. pylori from five North American rivers of different size and with different land use characteristics. Primers designed to amplify genes specific to Helicobacter spp. and H. pylori were evaluated for their efficacy in detection and quantification in environmental samples. Helicobacter spp. were detected in 18/33 (55%) of river samples. H. pylori was detected in 11/33 (33%) of river samples. FIB were found in 32/33 (96%) of river samples. When FIB abundance exceeded USEPA water quality standards for single samples, Helicobacter or H. pylori were detected in 7/15 (47%) cases. No numerical correlation was found between the presence of FIB and either Helicobacter spp. or H. pylori. This suggests that the presence of FIB will be of limited use for detection of Helicobacter spp. or H. pylori by public health agencies.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Detection of Helicobacter pylori and fecal indicator bacteria in five North American rivers.
Series title:
Journal of Water and Health
Volume
3
Issue:
4
Year Published:
2005
Language:
English
Publisher:
IWA Publishing
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Journal of Water and Health
First page:
405
Last page:
422
Number of Pages:
18