| Abstract: | A wetland sediment-derived microbial consortium (WBC-2) was developed by the US Geological Survey and propagated in vitro to large quantities by SiREM Laboratory for potential use in bioaugmentation applications. On the basis of bench-scale tests, the consortium could completely dechlorinate 1,1,2,2-tetrachloroethylene, tetrachloroethylene, trichloroethylene, 1,1,2-trichloroethane, cis- and trans-1,2-dichoroethylene, 1,1-dichloroethylene, 1,2-dichloroethane, and vinyl chloride in culture medium. Batch microcosms were carried out under anaerobic conditions in culture medium with neutral pH and with pH adjusted from acidic (pH 4, 5, and 6) to alkaline (pH 8 and 9). To evaluate oxygen sensitivity of WBC-2, an aliquot was removed from an anaerobic culture vessel and poured into smaller containers on the bench top where a series of oxygen exposures were applied to the culture by bubbling ambient air through the culture at a rate of ??? 100 mL/min. Chlorinated methanes tended to inhibit activity of a wide range of microorganisms. Although toxicity effects from CT addition were observed with WBC-2 in liquid culture at 3 mg/L concentration, WBC-2 in the columns could maintain degradation of CT and chloroform (CF) and of the chlorinated ethanes and ethylenes at CT and CF concentrations of 10 and 20 mg/L, respectively. This is an abstract of a paper presented at the Proceedings of the 8th International In Situ and On-Site Bioremediation Symposium (Baltimore, MD 6/6-9/2005). |
| Genre: | Conference Paper |
| ProdID: | 70027375 |
| Citation Author: | Lorah, M. M.; Majcher, E.; Jones, E.; Driedger, G.; Dworatzek, S.; Graves, D. |
| Citation Contributing Office: | |
| Citation Datum: | |
| Citation Day: | |
| Citation Edition: | |
| Citation Editor: | |
| Citation End Page: | 1449 |
| Citation Issue: | |
| Citation Keywords: | |
| Citation Language: | English |
| Citation Larger Work Title: | Proceedings of the 8th International In Situ and On-Site Bioremediation Symposium |
| Citation LatN: | |
| Citation LatS: | |
| Citation LonE: | |
| Citation LonW: | |
| Citation Month: | |
| Citation No Pagination: | |
| Citation Number Of Pages: | 2 |
| Citation Online Only Flag: | |
| Citation Phsyical Description: | |
| Citation Projection: | |
| Citation Public Comments: | |
| Citation Publisher: | |
| Citation Series: | |
| Citation Series Code: | |
| Citation Series Number: | |
| Citation Search Results Text: | Physiological characterization of a broad spectrum reductively dechlorinating consortium; 2005; Conference Paper; Proceedings of the 8th International In Situ and On-Site Bioremediation Symposium; Lorah, M. M.; Majcher, E.; Jones, E.; Driedger, G.; Dworatzek, S.; Graves, D. |
| Citation Start Page: | 1448 |
| Citation Volume: | 3 |
| Citation Year: | 2005 |
| Type: | citation/reference |
| Text: | Physiological characterization of a broad spectrum reductively dechlorinating consortium; 2005; Conference Paper; Proceedings of the 8th International In Situ and On-Site Bioremediation Symposium; Lorah, M. M.; Majcher, E.; Jones, E.; Driedger, G.; Dworatzek, S.; Graves, D. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| Date Other: | Sat, 1 Jan 2005 00:00 -0600 |
| Publisher: | |
