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Physiological characterization of a broad spectrum reductively dechlorinating consortium

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Abstract

A wetland sediment-derived microbial consortium (WBC-2) was developed by the US Geological Survey and propagated in vitro to large quantities by SiREM Laboratory for potential use in bioaugmentation applications. On the basis of bench-scale tests, the consortium could completely dechlorinate 1,1,2,2-tetrachloroethylene, tetrachloroethylene, trichloroethylene, 1,1,2-trichloroethane, cis- and trans-1,2-dichoroethylene, 1,1-dichloroethylene, 1,2-dichloroethane, and vinyl chloride in culture medium. Batch microcosms were carried out under anaerobic conditions in culture medium with neutral pH and with pH adjusted from acidic (pH 4, 5, and 6) to alkaline (pH 8 and 9). To evaluate oxygen sensitivity of WBC-2, an aliquot was removed from an anaerobic culture vessel and poured into smaller containers on the bench top where a series of oxygen exposures were applied to the culture by bubbling ambient air through the culture at a rate of ??? 100 mL/min. Chlorinated methanes tended to inhibit activity of a wide range of microorganisms. Although toxicity effects from CT addition were observed with WBC-2 in liquid culture at 3 mg/L concentration, WBC-2 in the columns could maintain degradation of CT and chloroform (CF) and of the chlorinated ethanes and ethylenes at CT and CF concentrations of 10 and 20 mg/L, respectively. This is an abstract of a paper presented at the Proceedings of the 8th International In Situ and On-Site Bioremediation Symposium (Baltimore, MD 6/6-9/2005).

Additional Publication Details

Publication type:
Conference Paper
Publication Subtype:
Conference Paper
Title:
Physiological characterization of a broad spectrum reductively dechlorinating consortium
ISBN:
9781574771527
Volume
3
Year Published:
2005
Language:
English
Larger Work Title:
Proceedings of the 8th International In Situ and On-Site Bioremediation Symposium
First page:
1448
Last page:
1449
Number of Pages:
2
Conference Title:
8th International In Situ and On-Site Bioremediation Symposium
Conference Location:
Baltimore, MD
Conference Date:
6 June 2005 through 9 June 2005