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Bi-parentally inherited species-specific markers identify hybridization between rainbow trout and cutthroat trout subspecies

Molecular Ecology Notes

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DOI: 10.1046/j.1471-8286.2003.00554.x

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Abstract

Eight polymerase chain reaction primer sets amplifying bi-parentally inherited species-specific markers were developed that differentiate between rainbow trout (Oncorhynchus mykiss) and various cutthroat trout (O. clarki) subspecies. The primers were tested within known F1 and first generation hybrid backcrosses and were shown to amplify codominantly within hybrids. Heterozygous individuals also amplified a slower migrating band that was a heteroduplex, caused by the annealing of polymerase chain reaction products from both species. These primer sets have numerous advantages for native cutthroat trout conservation including statistical genetic analyses of known crosses and simple hybrid identification.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Bi-parentally inherited species-specific markers identify hybridization between rainbow trout and cutthroat trout subspecies
Series title:
Molecular Ecology Notes
DOI:
10.1046/j.1471-8286.2003.00554.x
Volume
4
Issue:
1
Year Published:
2004
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Molecular Ecology Notes
First page:
26
Last page:
29
Number of Pages:
4