| Abstract: | Renibacterium salmoninarum is an important salmonid pathogen that is difficult to culture. We developed and assessed a real-time, quantitative, polymerase chain reaction (qPCR) assay for the detection and enumeration of R. salmoninarum. The qPCR is based on TaqMan technology and amplifies a 69-base pair (bp) region of the gene encoding the major soluble antigen (MSA) of R. salmoninarum. The qPCR assay consistently detected as few as 5 R. salmoninarum cells per reaction in kidney tissue. The specificity of the qPCR was confirmed by testing the DNA extracts from a panel of microorganisms that were either common fish pathogens or reported to cause false-positive reactions in the enzyme-linked immunosorbent assay (ELISA). Kidney samples from 38 juvenile Chinook salmon (Oncorhynchus tshawytscha) in a naturally infected population were examined by real-time qPCR, a nested PCR, and ELISA, and prevalences of R. salmoninarum detected were 71, 66, and 71%, respectively. The qPCR should be a valuable tool for evaluating the R. salmoninarum infection status of salmonids. |
| Genre: | Article |
| ProdID: | 70028075 |
| Citation Author: | Chase, D. M.; Elliott, D. G.; Pascho, R. J. |
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| Citation End Page: | 380 |
| Citation Issue: | 4 |
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| Citation Language: | English |
| Citation Larger Work Title: | Journal of Veterinary Diagnostic Investigation |
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| Citation Number Of Pages: | 6 |
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| Citation Search Results Text: | Detection and quantification of Renibacterium salmoninarum DNA in salmonid tissues by real-time quantitative polymerase chain reaction analysis; 2006; Article; Journal; Journal of Veterinary Diagnostic Investigation; Chase, D. M.; Elliott, D. G.; Pascho, R. J. |
| Citation Start Page: | 375 |
| Citation Volume: | 18 |
| Citation Year: | 2006 |
| Type: | citation/reference |
| Text: | Detection and quantification of Renibacterium salmoninarum DNA in salmonid tissues by real-time quantitative polymerase chain reaction analysis; 2006; Article; Journal; Journal of Veterinary Diagnostic Investigation; Chase, D. M.; Elliott, D. G.; Pascho, R. J. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| Date Other: | Sun, 1 Jan 2006 00:00 -0600 |
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