| Abstract: | We used molecular genetic methods to determine the sex of 138 sea otters (Enhydra lutris) harvested from 3 regions of Alaska from 1994 to 1997, to assess the accuracy of post-harvest field-sexing. We also tested each of a series of factors associated with errors in field-sexing of sea otters, including male or female bias, age-class bias, regional bias, and bias associated with hunt characteristics. Blind control results indicated that sex was determined with 100% accuracy using polymerase chain reaction (PCR) amplification using primers that co-amplify the zinc finger-Y-X gene, located on both the mammalian Y- and X-chromosomes, and Testes Determining Factor (TDF), located on the mammalian Y-chromosome. DNA-based sexing revealed that 12.3% of the harvested sea otters were incorrectly sexed in the field, with most errors (13 of 17) occurring as males incorrectly reported as females. Thus, female harvest was overestimated. Using logistic regression analysis, we detected no statistical association of incorrect determination of sex in the field with age class, hunt region, or hunt type. The error in field-sexing appears to be random, at least with respect to the variables evaluated in this study. |
| Genre: | Article |
| ProdID: | 70028257 |
| Citation Author: | Scribner, K. T.; Green, B. A.; Gorbics, C.; Bodkin, J. |
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| Citation End Page: | 1032 |
| Citation Issue: | 3 |
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| Citation Language: | English |
| Citation Larger Work Title: | Wildlife Society Bulletin |
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| Citation Number Of Pages: | 6 |
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| Citation Search Results Text: | Verification of sex from harvested sea otters using DNA testing; 2005; Article; Journal; Wildlife Society Bulletin; Scribner, K. T.; Green, B. A.; Gorbics, C.; Bodkin, J. |
| Citation Start Page: | 1027 |
| Citation Volume: | 33 |
| Citation Year: | 2005 |
| Type: | citation/reference |
| Text: | Verification of sex from harvested sea otters using DNA testing; 2005; Article; Journal; Wildlife Society Bulletin; Scribner, K. T.; Green, B. A.; Gorbics, C.; Bodkin, J. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| URL (DIGITAL OBJECT IDENTIFIER): | http://dx.doi.org/10.2193/0091-7648(2005)33[1027:VOSFHS]2.0.CO;2 |
| Date Other: | Sat, 1 Jan 2005 00:00 -0600 |
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