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Effects of polychlorinated biphenyls, hexachlorocyclohexanes, and mercury on human neutrophil apoptosis, actin cytoskelton, and oxidative state

Environmental Toxicology and Pharmacology

By:
, , , and
DOI: 10.1016/j.etap.2006.03.005

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Abstract

Apoptosis, or programmed cell death, has been proposed as a biomarker for environmental contaminant effects. In this work, we test the hypothesis that in vitro assays of apoptosis are sensitive indicators of immunological effects of polychlorinated biphenyls, hexachlorocyclohexanes, and mercury on human neutrophils. Apoptosis, necrosis, and viability as well as the related indicators F-actin levels, and active thiol state were measured in purified human neutrophils after treatment with contaminants. Effective concentrations observed were 0.3 ??M (60 ??g/L) mercury, 750 ??g/L Aroclor 1254, and 50 ??M (14,500 ??g/L) hexachlorocylcohexanes. Concentrations of contaminants that induced apoptosis also decreased cellular F-actin levels. Active thiols were altered by mercury, but not organochlorines. Comparison of these data with levels of contaminants reported to be threats to human health indicate neutrophil apoptosis is a sensitive indicator of mercury toxicity.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Effects of polychlorinated biphenyls, hexachlorocyclohexanes, and mercury on human neutrophil apoptosis, actin cytoskelton, and oxidative state
Series title:
Environmental Toxicology and Pharmacology
DOI:
10.1016/j.etap.2006.03.005
Volume
22
Issue:
2
Year Published:
2006
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Environmental Toxicology and Pharmacology
First page:
179
Last page:
188
Number of Pages:
10