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A method for measuring total thiaminase activity in fish tissues

Journal of Aquatic Animal Health

By:
, , , , and
DOI: 10.1577/H03-083.1

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Abstract

An accurate, quantitative, and rapid method for the measurement of thiaminase activity in fish samples is required to provide sufficient information to characterize the role of dietary thiaminase in the onset of thiamine deficiency in Great Lakes salmonines. A radiometric method that uses 14C-thiamine was optimized for substrate and co-substrate (nicotinic acid) concentrations, incubation time, and sample dilution. Total thiaminase activity was successfully determined in extracts of selected Great Lakes fishes and invertebrates. Samples included whole-body and selected tissues of forage fishes. Positive control material prepared from frozen alewives Alosa pseudoharengus collected in Lake Michigan enhanced the development and application of the method. The method allowed improved discrimination of thiaminolytic activity among forage fish species and their tissues. The temperature dependence of the thiaminase activity observed in crude extracts of Lake Michigan alewives followed a Q10 = 2 relationship for the 1-37??C temperature range, which is consistent with the bacterial-derived thiaminase I protein. ?? Copyright by the American Fisheries Society 2005.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
A method for measuring total thiaminase activity in fish tissues
Series title:
Journal of Aquatic Animal Health
DOI:
10.1577/H03-083.1
Volume
17
Issue:
1
Year Published:
2005
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Journal of Aquatic Animal Health
First page:
82
Last page:
94
Number of Pages:
13