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Isolation and cross-familial amplification of 41 microsatellites for the brook charr (Salvelinus fontinalis)

Molecular Ecology Notes

By:
, , , , and
DOI: 10.1111/j.1471-8286.2005.00922.x

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Abstract

The brook charr (Salvelinus fontinalis; Osteichthyes: Salmonidae) is a phenotypically diverse fish species inhabiting much of North America. But relatively few genetic diagnostic resources are available for this fish species. We isolated 41 microsatellites from S. fontinalis polymorphic in one or more species of salmonid fish. Thirty-seven were polymorphic in brook charr, 15 in the congener Arctic charr (Salvelinus alpinus) and 14 in the lake charr (Salvelinus namaycush). Polymorphism was also relatively high in Oncorhynchus, where 21 loci were polymorphic in rainbow trout (Oncorhynchus mykiss) and 16 in cutthroat trout (Oncorhynchus clarkii) but only seven and four microsatellite loci were polymorphic in the more distantly related lake whitefish (Coregonus clupeaformis) and Atlantic salmon (Salmo salar), respectively. One duplicated locus (Sfo228Lav) was polymorphic at both duplicates in S. fontinalis. ?? 2005 Blackwell Publishing Ltd.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Isolation and cross-familial amplification of 41 microsatellites for the brook charr (Salvelinus fontinalis)
Series title:
Molecular Ecology Notes
DOI:
10.1111/j.1471-8286.2005.00922.x
Volume
5
Issue:
2
Year Published:
2005
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Molecular Ecology Notes
First page:
346
Last page:
351
Number of Pages:
6