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In situ hydrogen consumption kinetics as an indicator of subsurface microbial activity

FEMS Microbiology Ecology

By:
, ,
DOI: 10.1111/j.1574-6941.2007.00286.x

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Abstract

There are few methods available for broadly assessing microbial community metabolism directly within a groundwater environment. In this study, hydrogen consumption rates were estimated from in situ injection/withdrawal tests conducted in two geochemically varying, contaminated aquifers as an approach towards developing such a method. The hydrogen consumption first-order rates varied from 0.002 nM h-1 for an uncontaminated, aerobic site to 2.5 nM h-1 for a contaminated site where sulfate reduction was a predominant process. The method could accommodate the over three orders of magnitude range in rates that existed between subsurface sites. In a denitrifying zone, the hydrogen consumption rate (0.02 nM h-1) was immediately abolished in the presence of air or an antibiotic mixture, suggesting that such measurements may also be sensitive to the effects of environmental perturbations on field microbial activities. Comparable laboratory determinations with sediment slurries exhibited hydrogen consumption kinetics that differed substantially from the field estimates. Because anaerobic degradation of organic matter relies on the rapid consumption of hydrogen and subsequent maintenance at low levels, such in situ measures of hydrogen turnover can serve as a key indicator of the functioning of microbial food webs and may be more reliable than laboratory determinations. ?? 2007 Federation of European Microbiological Societies.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
In situ hydrogen consumption kinetics as an indicator of subsurface microbial activity
Series title:
FEMS Microbiology Ecology
DOI:
10.1111/j.1574-6941.2007.00286.x
Volume
60
Issue:
2
Year Published:
2007
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
FEMS Microbiology Ecology
First page:
220
Last page:
228
Number of Pages:
9