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Gene expression in the liver of rainbow trout, Oncorhynchus mykiss, during the stress response

Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics

By:
, , , , ,
DOI: 10.1016/j.cbd.2007.06.002

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Abstract

To better appreciate the mechanisms underlying the physiology of the stress response, an oligonucleotide microarray and real-time RT-PCR (QRT-PCR) were used to study gene expression in the livers of rainbow trout (Oncorhynchus mykiss). For increased confidence in the discovery of candidate genes responding to stress, we conducted two separate experiments using fish from different year classes. In both experiments, fish exposed to a 3??h stressor were compared to control (unstressed) fish. In the second experiment some additional fish were exposed to only 0.5??h of stress and others were sampled 21??h after experiencing a 3??h stressor. This 21??h post-stress treatment was a means to study gene expression during recovery from stress. The genes we report as differentially expressed are those that responded similarly in both experiments, suggesting that they are robust indicators of stress. Those genes are a major histocompatibility complex class 1 molecule (MHC1), JunB, glucose 6-phosphatase (G6Pase), and nuclear protein 1 (Nupr1). Interestingly, Nupr1 gene expression was still elevated 21??h after stress, which indicates that recovery was incomplete at that time. ?? 2007 Elsevier Inc. All rights reserved.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Gene expression in the liver of rainbow trout, Oncorhynchus mykiss, during the stress response
Series title:
Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics
DOI:
10.1016/j.cbd.2007.06.002
Volume
2
Issue:
4
Year Published:
2007
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
First page:
303
Last page:
315
Number of Pages:
13