| Abstract: | Mozambique tilapia Oreochromis mossambicus embryos were transferred from freshwater to seawater and vice versa, and short-term changes in the localization of three major ion transport proteins, Na+/K +-ATPase, Na+/K+/2Cl- cotransporter (NKCC) and cystic fibrosis transmembrane conductance regulator (CFTR) were examined within mitochondrion-rich cells (MRCs) in the embryonic yolk-sac membrane. Triple-color immunofluorescence staining allowed us to classify MRCs into four types: type I, showing only basolateral Na+/K +-ATPase staining; type II, basolateral Na+/K +-ATPase and apical NKCC; type III, basolateral Na+/K +-ATPase and basolateral NKCC; type IV, basolateral Na +/K+-ATPase, basolateral NKCC and apical CFTR. In freshwater, type-I, type-II and type-III cells were observed. Following transfer from freshwater to seawater, type-IV cells appeared at 12 h and showed a remarkable increase in number between 24 h and 48 h, whereas type-III cells disappeared. When transferred from seawater back to freshwater, type-IV cells decreased and disappeared at 48 h, type-III cells increased, and type-II cells, which were not found in seawater, appeared at 12 h and increased in number thereafter. Type-I cells existed consistently irrespective of salinity changes. These results suggest that type I is an immature MRC, type II is a freshwater-type ion absorptive cell, type III is a dormant type-IV cell and/or an ion absorptive cell (with a different mechanism from type II), and type IV is a seawater-type ion secretory cell. The intracellular localization of the three ion transport proteins in type-IV cells is completely consistent with a widely accepted model for ion secretion by MRCs. A new model for ion absorption is proposed based on type-II cells possessing apical NKCC. |
| Genre: | Article |
| ProdID: | 70031550 |
| Citation Author: | Hiroi, J.; McCormick, S. D.; Ohtani-Kaneko, R.; Kaneko, T. |
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| Citation End Page: | 2036 |
| Citation Issue: | 11 |
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| Citation Language: | English |
| Citation Larger Work Title: | Journal of Experimental Biology |
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| Citation Number Of Pages: | 14 |
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| Citation Search Results Text: | Functional classification of mitochondrion-rich cells in euryhaline Mozambique tilapia (Oreochromis mossambicus) embryos, by means of triple immunofluorescence staining for Na+/K+-ATPase, Na +/K+/2Cl- cotransporter and CFTR anion channel; 2005; Article; Journal; Journal of Experimental Biology; Hiroi, J.; McCormick, S. D.; Ohtani-Kaneko, R.; Kaneko, T. |
| Citation Start Page: | 2023 |
| Citation Volume: | 208 |
| Citation Year: | 2005 |
| Type: | citation/reference |
| Text: | Functional classification of mitochondrion-rich cells in euryhaline Mozambique tilapia (Oreochromis mossambicus) embryos, by means of triple immunofluorescence staining for Na+/K+-ATPase, Na +/K+/2Cl- cotransporter and CFTR anion channel; 2005; Article; Journal; Journal of Experimental Biology; Hiroi, J.; McCormick, S. D.; Ohtani-Kaneko, R.; Kaneko, T. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| URL (DIGITAL OBJECT IDENTIFIER): | http://dx.doi.org/10.1242/jeb.01611 |
| Date Other: | Sat, 1 Jan 2005 00:00 -0600 |
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