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Histological alternation and vitellogenin induction in adult rare minnow (Gobiocypris rarus) after exposure to ethynylestradiol and nonylphenol

Chemosphere

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, , , and
DOI: 10.1016/j.chemosphere.2006.05.071

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Abstract

Adult rare minnow (Gobiocypris rarus) were exposed to 0, 1, 5, and 25 ng/l (nominal concentrations) of 17??-ethynylestradiol (EE2) and 3, 10, and 30 ??g/l (nominal concentrations) of 4-nonylphenol (NP) under flow-through conditions for a period of 28 d. Low mortality was observed at 5 and 25 ng/l EE2 and the growth of fish reduced significantly at 25 ng/l EE2 compared to controls. However, the gonadosomatic indices (GSI) of male fish were significantly higher in 1 ng/l EE2 treatments and in 10 and 30 ??g/l NP treatments (p < 0.05). Renal somatic indices (RSI) of male fish in EE2 treatments were significantly higher than those in controls (p < 0.05). In contrast, significantly decreased GSI and RSI of female fish could only be observed in 5 and 25 ng/l EE2 treatments (p < 0.05). Hepatosomatic indices (HSI) of male fish were significantly higher in 25 ng/l EE2 treatments. However, significantly increased of HSI of female fish could only be observed in 1 ng/l EE2 treatments. Plasma vitellogenin (VTG) induction could be observed in males after exposed to different concentrations of EE2 and NP, and plasma VTG concentrations in females exposed to 5 and 25 ng/l EE2 were also significantly higher than in controls (p < 0.05). At level higher than 5 ng/l EE2 or 30 ??g/l NP, hepatic tissue and renal tissue impairment of males could be observed. The pathological male liver was associated with a hypertrophy of hepatocytes and damages to cellar structure and accumulated eosinophilic material. Renal tissue showed different pathological effects which was reflected by accumulated eosinophilic material, hemorrhages within the kidney tubules and hypertrophy of the tubular epithelia. Also at these levels of exposure, feminization of male fish could be noticed and parts of males manifested the testis-ova phenomenon. Ovaries of female rare minnow in 25 ng/l EE2 treatment group were degenerated. Therefore when exposed to EE2 and NP even at environmental observed concentrations, adverse effects could occur in the reproductive system of adult fishes. The observed hepatic tissue and renal tissue impairment should be due to the induction and accumulation of VTG in organs, especially in males. ?? 2006 Elsevier Ltd. All rights reserved.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Histological alternation and vitellogenin induction in adult rare minnow (Gobiocypris rarus) after exposure to ethynylestradiol and nonylphenol
Series title:
Chemosphere
DOI:
10.1016/j.chemosphere.2006.05.071
Volume
66
Issue:
3
Year Published:
2007
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Chemosphere
First page:
488
Last page:
495