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An inducible HSP70 gene from the midge Chironomus dilutus: Characterization and transcription profile under environmental stress

Insect Molecular Biology

By:
and
DOI: 10.1111/j.1365-2583.2008.00853.x

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Abstract

In the present study, we identified and characterized an inducible heat shock protein 70 (HSP70) from the midge Chironomus dilutus and investigated the transcriptional profile of the gene under baseline and environmentally stressful conditions. Using real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR), we observed increased expression of CD-HSP70-1 in response to both heat shock and copper stress. We also investigated the expression of this gene during midge development. All C. dilutus developmental stages expressed CD-HSP70-1 under normal conditions, although at extremely low levels. Phylogenetic analysis of the amino acid sequence demonstrated distinct clustering of this gene with inducible HSP70s from other insect species. ?? 2008 The Authors.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
An inducible HSP70 gene from the midge Chironomus dilutus: Characterization and transcription profile under environmental stress
Series title:
Insect Molecular Biology
DOI:
10.1111/j.1365-2583.2008.00853.x
Volume
18
Issue:
1
Year Published:
2009
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
Larger Work Title:
Insect Molecular Biology
First page:
87
Last page:
96