Induction of anti-viral genes during acute infection with Viral hemorrhagic septicemia virus (VHSV) genogroup IVa in Pacific herring (Clupea pallasii)

Hansen, J. D.; Woodson, J. C.; Hershberger, P. K.; Grady, C.; Gregg, J. L.; Purcell, M. K.

Abstract:

Infection with the aquatic rhabdovirus Viral hemorrhagic septicemia virus (VHSV) genogroup IVa results in high mortality in Pacific herring (Clupea pallasii) and is hypothesized to be a potential limiting factor for herring recovery. To investigate anti-viral immunity in the Pacific herring, four immune response genes were identified: the myxovirus resistance (Clpa-Mx), a major histocompatibility complex IB (named Clpa-UAA.001), the inducible immunoproteosome subunit 9 (Clpa-PSMB9) and the neutrophil chemotactic factor (Clpa-LECT2). Reverse transcriptase quantitative PCR (RT-qPCR) assays were developed based on these gene sequences to investigate the host immune response to acute VHSV infection following both injection and immersion challenge. Virus levels were measured by both plaque assay and RT-qPCR and peaked at day 6 during the 10-day exposure period for both groups of fish. The interferon stimulated genes (Clpa-Mx,-UAA.001, and-PSMB9) were significantly up-regulated in response to VHSV infection at both 6 and 10 days post-infection in both spleen and fin. Results from this study indicate that Pacific herring mount a robust, early antiviral response in both fin and spleen tissues. The immunological tools developed in this study will be useful for future studies to investigate antiviral immunity in Pacific herring. ?? 2011.

Genre:

Article

ProdID:

70032666

Citation Author:

Hansen, J. D.; Woodson, J. C.; Hershberger, P. K.; Grady, C.; Gregg, J. L.; Purcell, M. K.

Citation Contributing Office:

Citation Datum:

Citation Day:

Citation Edition:

Citation Editor:

Citation End Page:

267

Citation Issue:

Citation Keywords:

Citation Language:

English

Citation Larger Work Title:

Fish and Shellfish Immunology

Citation LatN:

Citation LatS:

Citation LonE:

Citation LonW:

Citation Month:

Citation No Pagination:

Citation Number Of Pages:

Citation Online Only Flag:

Citation Phsyical Description:

Citation Projection:

Citation Public Comments:

Citation Publisher:

Citation Series:

Citation Series Code:

Citation Series Number:

Citation Search Results Text:

Induction of anti-viral genes during acute infection with Viral hemorrhagic septicemia virus (VHSV) genogroup IVa in Pacific herring (Clupea pallasii); 2012; Article; Journal; Fish and Shellfish Immunology; Hansen, J. D.; Woodson, J. C.; Hershberger, P. K.; Grady, C.; Gregg, J. L.; Purcell, M. K.