| Abstract: | Aims: The aim of this study was to examine a rapid method for detecting Escherichia coli and enterococci in recreational water. Methods and Results: Water samples were assayed for E. coli and enterococci by traditional and immunomagnetic separation/adenosine triphosphate (IMS/ATP) methods. Three sample treatments were evaluated for the IMS/ATP method: double filtration, single filtration, and direct analysis. Pearson‘s correlation analysis showed strong, significant, linear relations between IMS/ATP and traditional methods for all sample treatments; strongest linear correlations were with the direct analysis (r = 0.62 and 0.77 for E. coli and enterococci, respectively). Additionally, simple linear regression was used to estimate bacteria concentrations as a function of IMS/ATP results. The correct classification of water-quality criteria was 67% for E. coli and 80% for enterococci. Conclusions: The IMS/ATP method is a viable alternative to traditional methods for faecal-indicator bacteria. Significance and Impact of the Study: The IMS/ATP method addresses critical public health needs for the rapid detection of faecal-indicator contamination and has potential for satisfying US legislative mandates requiring methods to detect bathing water contamination in 2 h or less. Moreover, IMS/ATP equipment is considerably less costly and more portable than that for molecular methods, making the method suitable for field applications. ?? 2009 The Authors. |
| Genre: | Article |
| ProdID: | 70032842 |
| Citation Author: | Bushon, R. N.; Brady, A. M.; Likirdopulos, C. A.; Cireddu, J. V. |
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| Citation End Page: | 441 |
| Citation Issue: | 2 |
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| Citation Language: | English |
| Citation Larger Work Title: | Journal of Applied Microbiology |
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| Citation Number Of Pages: | 10 |
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| Citation Search Results Text: | Rapid detection of Escherichia coli and enterococci in recreational water using an immunomagnetic separation/adenosine triphosphate technique; 2009; Article; Journal; Journal of Applied Microbiology; Bushon, R. N.; Brady, A. M.; Likirdopulos, C. A.; Cireddu, J. V. |
| Citation Start Page: | 432 |
| Citation Volume: | 106 |
| Citation Year: | 2009 |
| Type: | citation/reference |
| Text: | Rapid detection of Escherichia coli and enterococci in recreational water using an immunomagnetic separation/adenosine triphosphate technique; 2009; Article; Journal; Journal of Applied Microbiology; Bushon, R. N.; Brady, A. M.; Likirdopulos, C. A.; Cireddu, J. V. |
| URL (THUMBNAIL): | http://pubs.er.usgs.gov/thumbnails/outside_thumb.jpg |
| URL (DIGITAL OBJECT IDENTIFIER): | http://dx.doi.org/10.1111/j.1365-2672.2008.04011.x |
| Date Other: | Thu, 1 Jan 2009 00:00 -0600 |
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