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Tetranucleotide microsatellite loci from the black bear (Ursus americanus)

Molecular Ecology Resources

By:
, , ,
DOI: 10.1111/j.1755-0998.2008.02234.x

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Abstract

We describe primers and polymerase chain reaction conditions to amplify 21 tetranucleotide microsatellite DNA loci in black bears (Ursus americanus). We tested primers using individuals from two populations, one each in Georgia and Florida. Among individuals from Georgia (n = 29), primer pairs yielded an average of 2.9 alleles (range, one to four) and an average observed heterozygosity (HO) of 0.50 (range, 0.00 to 0.79). Among individuals from Florida (n = 19), primer pairs yielded an average of 5.7 alleles (range, one to 14) and an HO of 0.55 (range, 0.00 to 1.00). A comparison of previously developed markers with individuals from Georgia suggests that bear populations in Georgia and Florida have reduced allelic diversity relative to other populations. ?? 2008 The Authors.

Additional Publication Details

Publication type:
Article
Publication Subtype:
Journal Article
Title:
Tetranucleotide microsatellite loci from the black bear (Ursus americanus)
Series title:
Molecular Ecology Resources
DOI:
10.1111/j.1755-0998.2008.02234.x
Volume
9
Issue:
1
Year Published:
2009
Language:
English
Larger Work Type:
Article
Larger Work Subtype:
Journal Article
First page:
288
Last page:
291
Number of Pages:
4